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WebSep 9, 2024 · Be sure to wear gloves. Prepare a hot water bath (100°C). Place some water in a 600 mL or larger beaker and microwave or leave on a hot plate to boil. (This can take 15 minutes or more.) Combine 10 µL of each protein sample with 20 µL of Laemmli sample buffer/Loading Dye in labeled screw-top microcentrifuge tubes. s47 family law act 1996
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