Phospho stat3

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August undefined, 2024

WebApr 13, 2024 · Phosphorylated STAT3 dimers translocate to the nucleus and induce the transcription of target genes (POMC, SOCS3). SOCS3 inhibits the JAK2/STAT3 pathway by interacting with phospho-Y985 or JAK2 and acting as a feedback inhibitor of leptin signaling. PTP1B also inhibits leptin signaling through dephosphorylation of JAK2. WebApr 10, 2024 · The fluorescence of phosphorylation-STAT3 was significantly upregulated in the induction group, while decreased in addition of Stattic (Fig. 6A, F). The expression profile of osteogenic genes including ALPL, RUNX2 and BMP2 (Fig. 6B–D) and proteins including BGLAP (Fig. 6E) and ALP (Fig. 6G) was in consistent with phosphorylation-STAT3.

Role of STAT3 signaling pathway in breast cancer

WebSTAT3 Antibodies Antibodies that detect STAT3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Flow Cytometry. These antibodies target STAT3 in Human, Mouse, Rat, Non-human primate and Porcine samples. WebApr 11, 2024 · STAT3 is widely expressed in organs and tissues, and the phosphorylation of STAT3 motivates downstream genes related to proliferation and apoptosis, thus performing proliferation effects. A previous study has revealed that the motivation of STAT3 signaling promotes colonic mucus secretion in DSS-induced colitis in mice ( 28 ). imaginarium tech and toys

STAT3 phosphorylation at Ser727 and Tyr705 …

WebPVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under … WebApr 11, 2024 · STAT3 is widely expressed in organs and tissues, and the phosphorylation of STAT3 motivates downstream genes related to proliferation and apoptosis, thus … WebApr 14, 2024 · Mass spectrometry–based analyses demonstrated that salt-inducible kinase 1 (SIK1) binds AKT and undergoes AKT-mediated phosphorylation, which compromises SIK1 tumor-suppressive functions. As a result, AKT relieved the binding and repression of STAT3 by SIK1 in a phosphorylation-dependent manner, resulting in breast cell … imaginarium sticker book

Human/Mouse Phospho-STAT3 (Y705) Cell-Based ELISA

WebWestern blot - Phospho-STAT3-Y705 Rabbit pAb (AP0070) Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (AP0070) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. WebPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho … list of edmonton oilers goaliesWebBrilliant Violet 421™ anti-STAT3 Phospho (Tyr705) Antibody. Human whole blood was stimulated with (top) or without (bottom) IL-6 for 15 minutes, then treated with RBC Lysis/Fixation Solution, and permeabilized with True-Phos™ Perm Buffer, then stained with CD3 APC and STAT3 pY705 (clone 13A3-1) Brilliant Violet 421™. list of editorial magazines

"WebThis DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human and mouse STAT3 (Y705) in cell lysates. An immobilized capture antibody specific for STAT3 binds both phosphorylated and unphosphorylated STAT3. After washing away unbound material, a biotinylated detection … " - Phospho stat3

Phospho stat3

WebMar 14, 2013 · ATR-107 inhibits IL-21 induced phosphorylation and nuclear translocation of STAT3. Even though IL-21 activates several STAT proteins including STAT1, STAT3, STAT5a and STAT5b, the activation of STAT3 was studies since it is the most significant and sustained for this pathway [].To demonstrate that ATR-107 blocks the function of IL-21, … WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes

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WebApr 12, 2024 · This was evidenced by the fact that the reduced phosphorylation of STAT3 at the Y705 position by stattic has unleashed these pro-metastatic functions of the cancer cells, leading to the increased presence of CSCs and to an EMT-like phenotype in the cells. The negative regulatory roles of Y705-STAT3 on CSC generation was also evidenced by … WebSTAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in …

WebSTAT3: Products The STAT family of transcription factors, first described as key mediators of interferon signal transduction, are now recognized as signaling factors downstream of many cytokines, growth factors, and hormones. At … Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and …

WebPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb (Alexa Fluor ® 750 Conjugate) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Species Reactivity: Human, Mouse, Rat, Monkey WebSTAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the …

WebPhospho-STAT3 (Ser727) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. 2 Published Figures 15 References View all (92) STAT3 antibodies Catalog # 44-384G 100 µL USD 453.00 Check your price & availability Join Aspire™ for free antibodies Quantity

WebMay 7, 2015 · STAT3 (signal transducer and activator of transcription 3 [acute-phase response factor]) is a latent transcription factor that mediates extracellular signals such as cytokines and growth factors through interaction with polypeptide receptors at the cell surface.6STAT3 protein becomes transcriptionally activated primarily by tyrosine … list of edison diamond disc recordsWebDec 21, 2012 · Western blotting for STAT3 revealed detectable phosphorylation of Y705 and S727 of STAT3 (Fig. 1 B and C) in a large panel of BTSC cultures with diverse mutational … list of edmonton catholic schoolsWebApplications. 1/500. 1/2000 - 1/20000. Predicted molecular weight: 88 kDa.Can be blocked with STAT3 (phospho Y705) peptide (ab179551). Stimulation may be required to allow detection of the phosphorylated … list of edi transactions in healthcareWebMay 7, 2015 · The literature suggests a modulatory role for Ser727 phosphorylation in STAT3 transcriptional activation, presumably through the enhanced recruitment of … list of edmonton oilers draft picksWebThe STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting. During PD … imaginarium ticketsWebFeb 28, 2024 · The role of STAT3 in breast cancer progression. An illustration of the advances in our understanding of the STAT3 signaling pathways in breast cancer progression is shown in Fig. 2.A member of the IL-6 family of cytokines, Oncostatin M (OSM) can induce IL-6 upregulation and STAT3 phosphorylation to promote breast cancer … list of edm record labelsWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) Research with … list of edith wharton books