Phospho ampk antibody cst
WebAMPK is a heterotrimer consisting of a catalytic α-subunit and two regulatory subunits, β and γ. Each subunit exists as multiple isoforms (α 1, α 2, β 1, β 2, γ 1, γ 2, and γ 3 ), giving 12 different possible combinations of holoenzyme with different tissue distribution and subcellular localization ( 2 , 3 ). WebWestern blot analysis of Phospho-IRE1 alpha (Ser724) in HeLa cells. Samples were incubated in Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927) using a dilution of 2.0 µg/mL followed by an anti-rabbit HRP secondary antibody. Treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR.
Phospho ampk antibody cst
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WebThis antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. The AMPKalpha Antibody was generated using …
WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). WebThe primary antibody to LC3B (ab192890, Abcam, Cambridge, MA, UK) or Cleaved Caspase 3 (#9664, CST, MA, USA) was added dropwise to the sections and incubated overnight at 4 °C. Next day, slices were washed three times in PBS before incubation with secondary antibody (A0208, Bain-marie Biotech, Shanghai, China) for 1 h at room temperature.
WebAMPK signaling pathway; 浏览更多通路>> ... Phospho-CDK9 (Thr186) Antibody detects endogenous levels of CDK9 only when phosphorylated at Thr186. RRID: AB_2844504 引用格式: Affinity Biosciences Cat# AF4440, RRID:AB_2844504. 偶联: Unconjugated. WebAMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1) are meta- bolic kinases that co-ordinate nutrient supply with cell growth. AMPK negatively regulates mTORC1, and mTORC1 reciprocally phosphorylates S345/7 in both AMPK a-isoforms.
WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPKα. …
WebSep 4, 2014 · Western blot analysis using a phospho-specific antibody revealed a significant increase in phospho-Thr184-AMPK levels in head tissue lysates from ElavGS>UAS-AMPK flies upon RU486 ... Primary antibody, rabbit anti-DILP2 (a generous gift from Dr. Seung Kim), or anti-GFP (Cell Signaling Technology; D5.1, XP) was added 1:250 and incubated … how many sq miles is belizeWebPolyclonal Antibody for studying AMPK-alpha1 (Ser496) phosphate. Cited in 30 publications. Validated for Western Blotting. Highly specific and … how many sq miles is chicagoWebPhospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen … how many sq miles is chileWebAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody Description Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Host species Rabbit Specificity ab23875 recognises the phosphorylated forms of AMPK alpha 1 (T183) and AMPK alpha 2 (T172). Tested applications how many sq miles is berlinWebOct 19, 2024 · Since its inception, CST has become the world leader in the production of the highest quality activation-state and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine. how many sq miles is israelWebDescription: The AMPK and ACC Antibody Sampler Kit provides an economical means to investigate energy homeostasis and fatty acid synthesis within the cell. The kit includes … how did stanislavski get into actingWebPolyclonal Antibody for studying AP-2 mu2 (Thr156) phosphate. Cited in 9 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-AP2M1 (Thr156) Antibody (CST #3843) is ready to ship. how many sq miles is greater london